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Only EST sequences having an e-value of less than 1 × 10-7 to known or hypothetical proteins of A. thaliana were kept for further analysis.
The lower rate of evolution of yaaW sequences having an overlapped htgA gene is explained by the higher constraints of a DNA sequence coding for two genes.
The single run produced more than 168,900 distinct sequences with 27,290 sequences having an above cut-off BLAST result.
To avoid ambuiguites in the evaluation of predictions, we further restricted the sets to sequences having an unique mRNA cleavage site, documented spread on less 40 bp.
Sequences having an E-value up to 7.0e-200 were taken into consideration for further analysis to cover the maximum number of genes.
Similar peptide sequences were clustered by creating a link between any two peptide sequences having an identity above 30% over an amino acid span of at least 50 aa.
Similar(50)
Sequences having a purine/pyrimidine ratio < 1 were reversed.
But other sequences have an astounding poignancy.
The basketball sequences have an easy fluency, and the bebop-y music accompanying a writing sequence suggests the risky thrill of creativity.
The test image sequences have an image size of pixels.
These high quality sequences had an average read of 500 bps.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com