Sentence examples for sequences for the validation from inspiring English sources
Sequences for the validation experiments were diluted to 50 fM [27] and sent to Genewiz, Inc. (New Jersey).
Primer sequences for the validations are found in Supplementary Table 2. PCR products were used as templates for sequencing with BigDye® Terminator reagents (Life Technologies) on a 3730xl DNA Sequencing Analyzer (Life Technologies).
Additionally, 11 genomic replicates were sequenced for the validation experiment, including multiple colonies from the same plate (n = 4), multiple passages of an isolate (n = 4), and independent sequencing experiments (n = 4) from the same DNA source.
(TIFF 32 KB) 12284_2014_20_MOESM8_ESM.xlsx Additional file 8: Table S1.: Primer sequences used for the validation of DEGs.
The PCR primer sequences used for the validation of CD4 gene loci will be provided upon request.
HRM results were compared with sequencing results for the validation of HRM analysis in the detection of EGFR mutations.
The visual determination of the position and context of each new mutation strongly facilitates the choice of sequencing primers for the validation of the mutation.
Original versus predicted breeding values for interval between first and last insemination for the two methods (GBLUP and BSSVS) using the three types of data (BovineHD, ImputedHD, and imputed sequence) for the 2087 validation animals.
Original versus predicted breeding values for somatic cell score for the two methods (GBLUP and BSSVS) using the three types of data (BovineHD, ImputedHD, and imputed sequence) for the 2087 validation animals.
Original versus predicted breeding values for protein yield for the two methods (GBLUP and BSSVS) using the three types of data (BovineHD, ImputedHD, and imputed sequence) for the 2087 validation animals.
In an effort to highlight the entire molecular consensus characterizing the RF gene families, we provide in Table 1 the complete RF-defined molecular consensus patterns and their sequences used in this study for the validation of the retrieved gene sequences.
