Analysis of the small RNA profiles of EV samples revealed enrichment of sequences of the size expected for miRNAs, with a peak at approximately 20 nt.
They were obtained by calculation of genetic distances for each pair of sequences of the size 390 and 391 bp of free-swimming mature cercariae (isolates Sz3 and Sz11) and fragments of single sporocysts (isolates Sz12 and Sz43).
This justification is consistent with the previous finding that the deletion of the 400 kb sequence responsible for the size differences of the homologous chromosomes of "3" resulted in no phenotypic consequences [ 16].
The archetype WSSV isolate TH-96-II, which has a 6,436 bp insertion compared to WSSV-TW [8], was used as a reference sequence for determining the size of the deletion.
Nevertheless, it should be noted that all of these genes contain complete coding sequences, suggesting that 5' or 3' UTR sequences are responsible for the size discrepancy between the Northern-blot results and the UniGene database.
The FF-based system and the proposed system perform similarly in the coastguard sequence for the GOP size equal to 2 and 4. In all other cases, the proposed system has better performance.
Systematically higher classification rates indicate that a region showed differential activation patterns for the four sequences, and the size of the classification accuracy served as a measure of the strength of the sequence representation in that region.
As each of P0_Reads and P0_Reads tables account for sequencing errors, the size of these tables vary as the sequencing quality changes.
This takes constant user time of 0.9 user seconds to match 1000 sequences to reference panels up to size 10 000, but for 50 000 reference sequences the size of the stored u, v and d arrays (which were not compressed in this implementation) became larger than the available memory, resulting in an increase in system time from 0.2 to 15 s, and a smaller increase in user time to 1.7 s.
For the primer sequences, the size of the PCR products and the specific cDNA region amplified see [19].
