Sentence examples for sequences for the pyrosequencing from inspiring English sources

Additional file 1: Primer sequences for the pyrosequencing assays.

The Norwegian Sequencing Centre is acknowledged for the pyrosequencing.

The same individuals selected for the initial Sanger cycle sequencing were sequenced with the pyrosequencing assay.

For sequencing according to the pyrosequencing technology, biotinylated PCR amplicons were immobilized onto streptavidin-coated magnetic beads and denatured to produce single-stranded DNA by using a PSQ 96 Sample Prep Tool (Biotage AB, Uppsala, Sweden).

The set-up for the pyrosequencing assay was selected with the following sequence in 'Sequence to Analyze': TACAG A/TGAAA.

The set up for the pyrosequencing assay was selected with the following sequence in 'sequence to analyse': TACAG A/TGAAA.

Primers (515F-modified 5′-GTGYCAGCMGCCGCGGTAA-3′ and 927R-modified 5′-CCGYCAATTCMTTTRAGTTT-3′; see Osburn et al. 2011) included adapter sequences for pyrosequencing on the GSFLX Titanium platform of the Roche 454 Pyrosequencing technology.

Primer sets of three primers for each SNP were designed, one pair of primers for the PCR (one of which was biotinylated) and a sequencing primer for the Pyrosequencing reaction.

The primer sequences for pyrosequencing were as follows: 5'-GGTTTTTTGGGTTTTTTGAA-3' (forward), 5'-Biotin TGAGGGTTTTGATGGTAT-3' (reverse) and 5'-TTGAATTTTGTAGGATTT-3' (sequencing primer).

Primer sequences for pyrosequencing are indicated in Additional file 4. Methylation levels for the CpG site were assessed using Pyromark Q24 pyrosequencer (Qiagen).

Our previous protocol was also used to select high-quality 16S rRNA gene amplicon sequences (with >350 and <550 bases in lengths) and for their subsequent processing (i.e. alignment to a standard sense strand and removal of primer sequences for pyrosequencing).