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Primer sequences for the construction of vectors are provided in Supplementary Table S3.
Clustal W [ 40] was used to align multiple sequences for the construction of phylogenetic trees.
Since the algorithms used to align genome sequences for the construction of the consensus genome (MAFFT) and for the extraction of SNPs and indels (MUMmer) are different, we manually checked the result of indel calls in the MUMmer data, and indels greater than 5 bp were used for manual annotation.
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The primer sequences used for the construction of sgRNA expression plasmids are listed in Table S1.
The primer sequences used for the construction of PCFJ151-C30F12.4 are listed in Table S1.
The nearest-neighbours sequences used for the construction of the previous trees were obtained using the selected representatives of each OTU and the Aligner tool through the web service provided by the SILVA database [40].
The nearest-neighbour sequences employed for the construction of the previous trees were obtained using selected representatives of each OTU and the Aligner tool provided by the SILVA database [61].
The sequences used for the construction of the gene indices were generated from various diverse libraries that cover different treatments and stages of development (Table 2).
Multiple alignment performed on the whole set of putative NAC protein sequences served for the construction of a phylogenetic tree, which revealed that the family can be divided into 12 clades.
Overlapping EnvBF synthetic peptides (15-mers, overlapping by 11 aa) were designed based on the sequence of the Env protein from CRF12_BF reference strain ARMA159; the same sequence employed for the construction of the DNA and VV vectors, and custom ordered from JPT Peptide Technologies (Germany).
Our results demonstrate the value of selecting from the diversity of BMC shell building blocks found in genomic sequence data for the construction of novel compartments.
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