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Probably these two domains require additional muNS sequences for proper folding, proper spatial disposition, or both.
Furthermore, the normal appearance of Abd-B expression in A7/PS11 in this iab-6 class of revertants clearly demonstrates that the Fab-7 1 deletion does not affect essential sequences for proper iab-7 activity.
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Our findings therefore implicate selection acting both to maintain the integrity of the hairpin structure for proper biogenesis and to maintain miRNA sequence for proper target specificity.
However, the 5'UTR sequences still have ~5 to 30% nucleotide sequence variations among different serotypes so require more than one prototype sequence for proper identification and serotyping.
Each rDNA unit consists of a transcribed sequence and an external non-transcribed spacer (Hadjiolov 1985; Liau and Perry 1969) in which all the sequences necessary for proper RNA pol I transcription such as proximal promoters, spacer promoters and terminators are located (Hadjiolov 1985).
The single non-expressing-promoter construct (MINI3) may not have contained all the cis-regulatory sequences required for proper expression in the female gametophyte.
If indel events have occurred during the course of evolution of the molecular sequences being studied, it becomes necessary to align the corresponding homologous regions among the sequences for a proper site-by-site comparison among them, before phylogenetic analysis.
Of the three RPS10B promoter::cDNA fusions, RPS10B::B rescued most efficiently, however with a further reduction compared to the genomic construct, which could indicate a requirement to generate alternative transcripts, or for intronic or untranslated sequences for the proper control of RPS10B gene expression.
This suggests that the N-terminal portion of KIAA1199 functions as a cleavable signal sequence required for proper KIAA1199 translocation and KIAA1199-mediated HA depolymerization.
Recently, Kinefold was used in an RNA engineering context to design appropriate sequence contexts for proper folding of ribozymes and aptazymes engineered to control the mRNA degradation rate, and thus enzyme levels, in a biosynthetic pathway [ 11].
The results of this study suggest that many functional RNA sequences are optimized for proper folding.
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