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For differentiating these transformants at molecular level, thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) was performed to separate the T-DNA flanking sequences for identifying transgenic plants in event-specific detection.
Each isolate exhibited a different IGS sequence, demonstrating the utility of IGS sequences for identifying strain diversity (data not shown).
It would be helpful to have more explanation of exactly what criteria were used for choosing the set of sequences for identifying mutations and measuring dn/ds ratios.
In this study we show the effectiveness of integrating global gene expression array analysis with a bioinformatic approach aimed at detecting AML1-consensus sequences for identifying novel putative direct AML1-targets downregulated by AML1-MTG16 in 32D cells.
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In our estimate, considering the current state of sequencing capabilities and the small number of sequences necessary to identify the breakpoint, we can reliably multiplex up to ten samples in a single lane of the Illumina sequencer, making the sequencing costs much lower than those for whole genome sequencing for identifying cancer-specific recombination biomarkers.
These data highlight the power of whole-genome sequencing for identifying recurrent somatic alterations in cancer genomes that may be missed using other methods.
In this study, we demonstrated that T2-weighted imaging is the most effective MRI sequence for identifying anatomic correlates to 68Ga-PSMA-11 68Ga-PSMA-11 68Ga-PSMA-11ymph nodes, and DCE is most effective for identifying PET-avidd foci in the prostatic bed.
SBE is also a more sensitive technique than cloning and sequencing for identifying alleles.
We also acknowledge that the FISP sequence may not be the optimal MR sequence for identifying all the meniscal and bone lesions.
For the other two scaffolds, scaffold_480 and scaffold_160, we used only the reliable Pcdh-containing sequences as the anchor sequence for identifying overlapping cosmid and BAC clones by BLASTN search of the cosmid or BAC end databases [ 22].
The promoter regions of the selected genes comprising 910 bp upstream and 90 bp downstream sequences obtained from Plant Promoter Db were used as an input sequence for identifying cis-acting regulatory elements (CAREs).
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