The results highlight the promise of next generation sequencing for development of genomic resources for any organism.
> Identification of potential splice site was undertaken as an extension to the usability of data generated from 127 754 TUSs aligned against soybean genome sequence for development of genetic markers in pigeonpea.
According to the original sequences used for development of SSRs, SSRs were divided in two categories: genomic-SSRs, identified from random genomic sequences, and expressed sequence tag (EST -SSRs, idEST -SSRsfrom transcridentifiedequences.
In addition to the analysis of the rye genome composition, we used BAC end sequences for marker development.
Using next-generation deep sequencing we have produced a large EST dataset to allow for the selection of probe sequences for the development of the EMPV1 microarray platform.
The SNP list provides allele information, flanking sequences (for assay development and strand verification, etc) and links for validation information and source databases.
In recent years, several studies have highlighted the immense potential of polymorphisms in intron sequences for the development of markers for genetic mapping [ 25- 27].
We thus sequenced the positive BAC clones from the 1145, FAP1360A, and Huangzao4 BAC libraries to mask the repetitive sequences at the Scmv1 locus, in order to reveal single/low-copy sequences for marker development.
In conclusion, we present a broad overview of A. americanum genomics and contribute EST sequences for the development of tick genomics, functional annotation of tick genomic sequences, and enhancement of biological understanding of these major disease vectors.
We further evaluate the structural properties of the SSR loci associated with elevated numbers of length variants and assess the efficacy of BAC and EST library nucleotide sequences for the development of informative molecular markers.
