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TR density was defined by base pairs per megabase pairs (bp/Mbp), namely the length of detected TRs out of the total length of the sequences for detection.
To systematically evaluate the diagnostic performance of increased signal intensity within the resection cavity on fluid-attenuated inversion recovery (FLAIR) sequences for detection of progression in patients with glioma through performing a meta-analysis.
The primer sequences for detection of transgene-derived BRI1 expression are GFPSEQF: 5'-acaacatcgaagacggcggcgtg-3' (forward) and KH002: 5'-cagtaggattgtggtgtgtgcgc-3' (reverse).
for 22 cycles, and post-elongation at 72°C for 7 min. The primer sequences for detection of endogenous BRI1 expression are 15F7: 5'-tgcgatggatacgcatttaa-3' (forward) and BRI1 3'UTR: 5'-tcggactgacccttagatg-3' (reverse).
In addition, Li et al 10 also showed the feasibility of amplification using peptide-nucleic-acid, which demonstrates high binding affinity to specific sequences for detection of paternally-inherited mutations in β-thalassaemia.
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Recent surveillance reports applying PCR and sequencing for detection and identification of rickettsiae have also detected R. typhi DNA in X. cheopis, Leptosylla segnis, and Ctenocephalides felis fleas in different parts of the world (2, 5, 8 ).
Bodian, D. L. et al. Utility of whole-genome sequencing for detection of newborn screening disorders in a population cohort of 1,696 neonates.
To assess hsp65 gene sequencing for detection and species identification of genus Nocardia from ocular isolates.
In the TZ, T2W imaging is now recognised as the most important pulse sequence for detection of PCa because of overlap between TZ cancer and stromal BPH on functional imaging (see Interpretive pitfalls, stromal BPH) [58].
However, the sensitivity of dideoxy-sequencing for detection of heteroplasmy quickly falls off, i.e. 75∶25 mixture of two sequences are detectable, but 88.5∶12.5 mixture are usually not.
Furthermore, the application of whole genome and transcriptome sequencing for detection of the human tissue-specific A-to-I RNA editing would be even more costly.
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