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Since DNA sequences differing in a single base often have different melting temperatures (Tm), discrimination between homozygous wild-type and homozygous mutant samples can be achieved by comparing the Tm values of test samples with those of wild-type controls.
The semi-quantitative technique is based on the amplification of short, overlapping sequences differing in size.
Zebra finches were trained with sequences differing in transitional and positional information and next tested with novel strings sharing positional and transitional similarities with the training strings.
To test whether or not short-term statistics in the delivery of calls are important to their neural processing, we compared response strengths of rare and common calls across sequences, differing in their call delivery rate.
In addition, SYBR Green I sequence specificity was investigated, using a set of sequences differing in length and base composition.
To check for specificity of this assay, control experiments were run using probe sequences differing in a single-base substitution within the repeat monomer ((TTA CG n and (TTTA CG n).
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However, only a subset of the secreted variants was detected within extracts of mammalian cells exposed to the same bacterial strains, suggesting that the different secretion sequences differ in their translocation efficiencies.
The obtained coding sequences differed in both length (292 445 aa) and sequence (56 70 aa differences) but formed a robust clade in the tree (not shown).
But the two sets of sequences differed in two positions.
These sequences differ in length and exhibit very low sequence identity to the target sequence of S. macrospora [44].
Therefore, we analysed whether the two groups of sequences differed in their amino acid composition (Figure 3A).
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