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This stitching process relies on overlapping sequences containing one or more SNVs and requires a reference genome to map the reads.
We found 255 sequences containing one or more SSR motifs.
Frequency of cSSR refers to kilobase pairs of cDNA sequences containing one SSR.
We identified 255 sequences containing one or more microsatellite motifs in the library screen.
Of the 64 sequences containing one or more SSR, 50 (78%) were amenable to primer design.
This was applied to sequences of homozygotes or sequences containing one polymorphic site.
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Ligamers targeting outermost sequences contain one region of complementarity and primer sequences for subsequent amplification.
Out of these, 4506 sequences contained one or more predicted open reading frames of at least 75 amino acids.
All BrIPT protein sequences contained one or two conserved regions that belonged to the P-loop NTPase superfamily (cl09099).
The great majority of the obtained sequences contained one or more internal stop codons or (rarely) a frameshift mutation (Table 1).
All sequences contained one consensus transmembrane region predicted by all of the four servers used, except for CpsA, which was predicted to have three transmembrane helices.
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