We designed four real-time PCR assays that amplified 18S ribosomal RNA gene sequences common to various plants at lengths of approximately 100, 200, 400, and 800 base pairs (bp).
After some high-tech sequencing--which now can be done at breathtaking speeds--and double checking for sequences common to other tumors, the team came up with hundreds of candidates.
Genes involved in RNA metabolism, i.e., genes whose products synthesize, degrade, or interact with RNA, are among the most highly conserved sequences common to all known genomes, and provide insights into an early stage in cell evolution during which RNA played a much more conspicuous biological role (Tekaia et al. 1999, Delaye and Lazcano 2000, Anantharaman et al. 2002).
Sequential BLASTn analysis resulted in two datasets, one containing sequences common to all Non-CF metagenomes and the other with sequences common to CF metagenomes.
Universal primers are those sequences common to the archaeal and eubacterial predicted primers.
In addition, there were no sequences common to lymphocytes and neutrophils and most of them were sometimes hypermethylated in NPTs.
Sequential BLAST analysis identified 31,413 sequences common to all CF viromes, and 12,824 of these did not appear in any of the Non-CF viromes.
Sequences common to NE strains JGS4143 and CP4, but absent from nine reference genomes, were identified via MUMmer v3.21, Bedtools v2.1 [76], and custom perl scripts.
Positive dataset training sequences common to these sequences were excluded; leading to an independent dataset of 54 cyclin sequences (Dataset S3).
We designed seven RNAi duplexes targeting sequences common to both SYT/L and SYT/S (panSYT) mRNAs and two RNAi duplexes specific for SYT/L mRNA (Figure S1).
V2C18 contains a 192-aa Domain of Unknown Function-959 (DUF-959) at its N-terminus, followed by sequences common to all variants [8].
