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Different classes of organisms (e.g. prokaryotes, unicellular eukaryotes, and multicellular eukaryotes) as well as different genomes structures (e.g. codifying sequences, introns, and "junk" sequences) can present huge differences in constraints.
In short, our results, although based on a limited number of gene sets, render some support to the idea that regulatory and protein-coding sequences can present different rates of substitution and adaptive changes responding to their own evolutionary dynamics.
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In summary, paired-genome sequencing in cancer can present us with a highly accurate view of how the cancer genome has evolved from a normal cell's DNA.
However, genomes with more complex repeat structure and lower sequencing coverage depths, can present challenges to the estimation approach presented here..In principle, this 21-mer abundance analysis can be applied to any sequencing methodology; however, there are some additional limitations.
Collectively, these results suggest a shared molecular basis among fragile sites, indicating that the DNA sequences within these regions can present significant difficulties to the replication machinery, and require the activation of DNA damage checkpoint proteins.
Different HLA alleles have different predilections for the sequence of peptides they can present to T cells.
Some of these features can present major problems during the sequence-finishing process; the most common being gaps (missing sequence) of various types and characteristics, and sequence misassemblies.
But it can present problems.
Plasmids may be integrated into the genome and thereby the plasmid sequences can be present in the assemblies surrounded by two different sequence environments (plasmid only sequences or adjacent genome parts), making the integration site a low coverage region.
Multiple identical copies of duplicated FRT-like sequences can, in theory, present a challenge for targeting unique FRT-like sequences of interest if the duplicated sequences have a high degree of homology to a chosen unique FRT-like site.
Schaffner's reply to the multiple realization objection (1993, 463 6) holds that the same DNA sequence can be present in many individuals, so it is not just a token phenomenon that is studied by molecular biology; molecular generalizations apply to restricted types.
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CEO of Professional Science Editing for Scientists @ prosciediting.com