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Depending on the number of possible POS tags and the model order, the number of different POS sequences can increase beyond a feasible amount.
Moreover, all constructs containing CpG sequences can increase transcriptional activity.
The 5' end of most viruses has also proved difficult to complete and we have found that the addition to the RT reaction of degenerate oligos based on conserved 5' sequences can increase coverage.
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As can be observed in Figure 19, the ML schemes for the Wineyard sequence can increase the coverage for the min quality category but also show a high loss for the max quality.
In addition, fusion of the XTEN sequence can increase the solubility and stability of the attached peptide, while simultaneously enabling recombinant production and improved manufacturing.
It is well-known that a stronger turn-promoting sequence can increase the stability of β-hairpins.
MARKs contain numerous kinase-regulated sites [ 39, 40] and, for example, phosphorylation at the Thr site (of MARK4 sequence) can increase MARK activity >50 times [ 33].
Allowing a mismatch in miRNA seed sequence can increase the regulation of diverse targets leading to the coexpression of these target genes.
An extra a few cycles using Tm pp-5 as the annealing temperature can increase the synthesis of the full-length plasmid and placing the mutation sites within the primer-primer overlap sequence can increase the mutation efficiency.
These motifs often function in protein scaffolding, and their multivalency (tendency to occur multiple times in one sequence) can increase the avidity of interactions and promote phase transition (see section 9.2).
Although further technical refinements, such as controlled hydrolysis of RNA samples before cDNA synthesis and paired-end or di-tags sequencing, can increase significantly the number and diversity of sequences which can be annotated, our protocol has proved to be very effective in detecting rare or novel transcriptional events.
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