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A model is proposed in which adjacent and overlapping sequences can function as a binding site, forming a short duplex with the sequence at the circularization site and thus directing circularization and reverse circularization.
To test whether these sequences can function as the cis-regulatory elements, we generated various promoter deletion constructs (Fig. 2B).
It is not clear whether these sequences can function similarly in the context of the intact protein, however.
Taken together, these results clearly show that the intergenic HFRs from the murine Hox clusters selectively block the LCR enhancer from acting on γ-neo reporter gene, indicating that these sequences can function as boundary elements.
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For one thing, the sequence can function like a reference library to be consulted as new knowledge comes to light.
Wang et al. [ 59] demonstrated that the human ortholog of this sequence can function as an enhancer of both LWS and MWS opsin expression in mice.
As was found for segmental duplications, also MSRs may be unstable DNA structures and therefore contain certain sequences that can function as a hotspot of structural genomic rearrangements.
Moreover, the new system provides an alternative approach to selectively clone large fragments of bacterial genomes [ 18] that are poor in sequences that can function as ARSs in yeast [ 14].
An Arg-Gly-Asp (RGD) sequence that can function for cell adhesion is found in a tight turn on an exposed loop.
However, we cannot exclude that the deletion has created a new regulatory element, or relocated an existing sequence that can function as a tissue-specific silencer.
However, they can be secreted and thus belong to the group of proteins that do not contain a signal sequence but can function outside cells [ 5].
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