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Our observations, based on replicated, standardized cultures, show that cercomonad strains with identical 18S rDNA but different ITS1 sequences can differ in several respects: salinity tolerance, propensity to form cysts, vigour in standard non-saline media (growth density and persistence over time), size and basic cell morphology (Table 2).
We also demonstrate that strains with identical 18S but different ITS1 rDNA sequences can differ significantly in terms of morphological and important physiological characteristics, providing strong additional support for global protist biodiversity being significantly higher than previously thought.
It should be noted that mammalian DLX6 sequences can differ in as many as 9 positions from the canonical sequence.
The AARE sites have a 9 bp core element (5'-A/GTTG/TCATCA-3') but the sequences can differ by one or two nucleotides between genes.
However, despite very well conserved residues, the homology between amino acid sequences can differ from one protein to another.
Because the probes on the rice genome array were designed for mainly japonica, their target sequences can differ from the target RNAs derived from indica at many positions.
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One possible explanation is that PCR-based MS-HRM assays cover only approximately 100 bp, whereas the region with aberrant methylation called on the microarray can span large genomic regions and the methylation status within those sequence can differ [ 11].
As the beads are randomly distributed on the plate, and the DNA on each bead is amplified and sequenced independently, the final length and error content of the sequence read can differ, but in all cases, the starting position of the read will be identical for all duplicates.
Of course, the genomics community, with its longer history of data sharing, has some strong positive examples where data sharing has benefitted researchers, but sequencing data can differ greatly from species abundance or behavioural trait data, for example.
Even closely related isolates belonging to the same clone or sequence type (ST) can differ in their capacity to cause disease [23].
While ICK toxin sequences can also differ dramatically among spiders, clades of more closely related sequences also occur in some spider species, and likely represent more recent, species-specific gene family diversification [ 45].
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