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The LA kernel measures the similarity between two sequences by adding up the scores obtained from local alignments with gaps of the sequences.
The numbers of potential sites overlapping each nucleotide position were summed up for individual bases and then the density of potential binding sites for each 2 kb window along the 150 kb upstream of the Olig2 coding sequences by adding up the number of sites at each base in the window.
For that, we modified the original RefSeq sequences by adding nucleotide-level substitution/indel errors.
SIFT selects sequences by adding the most similar sequence from a protein database iteratively to a growing collection until conservation in the conserved regions decreases.
Azzabou and Paragios [ 37] denoised ultrasound sequences by adding temporal filtering within the sequence, assuming that the image content does not vary to a large extend from frame to frame.
We used previously validated survivin and cyclin B1 siRNA sequences [ 33, 34], designed sticky siRNAs with these sequences by adding 3' complementary overhangs and first tested their silencing efficiency both at the mRNA and protein levels in B16-F10 cells.
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Although the plasmid initially contained the dCas9 sequence, which lacks endonuclease activity, we obtained the Cas9 sequence by adding point mutation in the primers.
Primer pairs were modified for sequencing by adding the forward Illumina Nextera adapter, a two basepair "linker" sequence, a unique 7-bp barcode sequence at the 5′ end of the forward primer, and the appropriate reverse Illumina Nextera adapter and linker sequence at the 5′ end of the reverse primer18.
In order to increase the throughput of barcode sequencing after shRNA screening, we established a strategy for multiplex barcode sequencing by adding 8-nt index sequences to the PCR primers used for barcode amplification, allowing us to distinguish each sample in the same analysis (see Supplementary Fig. S1).
In the second version, (right panel, Fig. 3a), the acceptor vector encodes a single loxPsym site, inserted in the URA3 coding sequence by adding two base pairs (TG) to the 3′ end of the 34 bp loxPsym site, resulting in an in-frame insertion of 36 bp (Supplementary Fig. 10).
Obviously, modifying the sequence by adding a sequence optimised peptide tag also avoids expression problems associated with the inition of translation.
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