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Here, we describe crystal structures of human and Drosophila melanogaster eIF4E-eIF4G complexes, which unexpectedly reveal that the eIF4G auxiliary sequences bind to the lateral surface of eIF4E, using a similar mode to that of 4E-BPs.
The fluorescently labeled target sequences) bind to the probe sequences (hybridization) and generate a signal whose strength is measured.
It arises due to spurious effects such as incomplete hybridization where probe sequences bind to only partially complementary targets or due to other optical effects.
RGD sequences bind to integrin receptors and, when immobilized on a surface, can lead to cell adhesion and spreading, a key feature for tissue engineering scaffolds.
The stereochemical hypothesis holds that the assignment of anticodons/codons to amino acids was based on stereochemical affinity, that is, specific anticodon/codon sequences bind to specific amino acids [ 8].
The bulged miR-9 antisense sequences bind to endogenous mature miR-9, serving as a miR-9 sponge to inhibit miR-9 cellular activity without altering endogenous miR-9 expression.
Similar(53)
HRP chemiluminescence detection was used to visualize biotinylated sequences bound to each array.
The possibility to screen, localize, and characterize the whole human genome for sequences bound to HMGA2, can help to understand in which way HMGA2 is associated with different biological processes.
As a result, the amounts of the two allelic sequences bound to the spot should be proportional to the initial amounts in the PCR product.
Because ChIP-Seq is only a slight enrichment (not a purification) for sequences bound to the protein of interest [ 15], it is notoriously noisy.
In this study we identified some RNA sequences bound to TLS with high specificity (for example, at the fragments 1 and 7), other RNA sequences bound to TLS with low specificity (at the fragments 3 and 4), inspiring that TLS binds to these two categories of RNA fragments through distinctive surface(s) of the molecule.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com