Exact(1)
Hence, an alternative methodology for genome editing would (1) facilitate the integration of such a homology cassette nearby the locus of interest, using a methodology different from donor-dependent homologous recombination; and (2) use single-strand annealing to resolve this cassette, leaving mutations or other functional sequences behind.
Similar(58)
The sequence behind the pits – fast, narrow.
You do get a lot of their signature style – blaring faux brass, chattery synth bass and cantering drum machine – to which time has not been terribly kind, though there are some deft touches: the weird, seasick chord sequence behind the "tay-tay-tay" hook of Mel and Kim's Respectable, the Northern Soul melody of Lonnie Gordon's Happenin' All Over Again.
Now that we have almost 60 years of DNA-based molecular genetics and genome sequencing behind us, a different picture has emerged.
Watch the Marco Polo Title Sequence behind the scenes film below and find out more about the Netflix series written by John Fusco and produced by The Weinstein Group here.
The pMTT1-BICH3 construct designed by Gorovsky's laboratory is able to target the IAG48[G1] sequence behind the powerful inducible metallothionein MTT1 promoter to the BTU1 coding region by biolistic transformation [16].
Our aim was to engineer roX1 without leaving vector or P-element sequence behind.
After initial targeting, both the MiMIC-based method and attP-CRISPR/Cas9 leave exogenous vector and/or marker sequence behind, and we provide a methodology to remove the unwanted vector and marker sequences that are present following the initial targeting event.
Sequencing the entire EST clone from the I.M.A.G.E consortium [ 3], which was supplied by the Resource Center of the German Human Genome Project at the Max-Planck-Institute for Molecular Genetics, confirmed the genomic sequence behind the coding region for transmembrane domain six.
We placed the pre-miR-E1-1 or pre-miR-E2-4 coding sequence behind a potent Pol II promoter CAG and in the 3'UTR of the RFP gene.
In this study, rBmNPV-hPRR, which displays a native form of human prorenin receptor (hPRR) with FLAG peptide sequence behind its signal peptide sequence on its own surface [ 15], was produced in silkworm larval hemolymph; purified by ultracentrifugation and two different types of SEC.
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