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In left SPL, gene carriers displayed greater activations with the simple motor sequence, whereas controls showed equal or greater activations of the same region with complex motor sequences (bar plots in Fig. 4).
An alignment of eukaryotic NrdJ sequences together with the sequences for the two structurally solved NrdJs (monomeric L. leichmannii and dimeric T. maritima respectively), and a handful of other putatively monomeric and dimeric NrdJ sequences, indicates that all eukaryotic NrdJ sequences, bar that from T. vaginalis, have the insert particular to monomeric NrdJs (figure 8).
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For multiplexing different samples in a single sequence run and a single lane on the Illumina Genome Analyzer, we use index sequences (bar-coding).
These sequences were aligned along with our previously published C. c. recticrista mtDNA sequences (Bar-Yaacov, Arbel-Thau, et al. 2012), as well as with four available non-Israeli C. chamaeleon sequences (Macey et al. 2008).
Following the ideas underlying PPCs, see, e.g., [29], at each time instant t, and for current state x t, the controller sends the entire optimizing sequence, (bar {u}_{t}), to the actuator node.
My favorite Bond action sequence bar none occurs in From Russia With Love, where Connery takes on assassin Robert Shaw in a closed train compartment.
In all parts of the figure vertical lines within the sequence bars indicate uHS specific sequence.
We applied parallel mutant phenotyping by barcode sequencing (Bar-seq) to assay pooled haploid deletion mutants as they aged together during long-term quiescence.
In order to investigate multiplexing performance of the modified library preparation method, the individual sequencing libraries were constructed with a unique 6 nucleotide sequence bar-code positioned in the reverse primer during library pre-amplification.
Barcode analysis by sequencing ("Bar-seq") counts each barcode in a complex sample (Robinson et al. 2014), and outperforms barcode detection by microarray hybridization, offering improved sensitivity, dynamic range, and greater limits of detection.
Synthetic long reads are made by sequencing bar-coded ∼10 kb genomic fragments with standard Illumina short-reads, performing a local assembly of these 10-kb fragments into synthetic long reads, and then feeding them into a standard assembler (Voskoboynik et al. 2013; McCoy et al. 2014).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com