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Predotar is particularly good at distinguishing mitochondrial and plastid targeting sequences as previously reported [33].
Affymetrix GeneChip probes were mapped to cUTR and aUTR sequences as previously described [18].
As expected, NF-Y does not bind the mutated consensus sequences, as previously reported [24], [49].
Individual clones were compared to consensus sequences as previously described [12].
The resulting recombinant PCR product was used to transform A. pleuropneumoniae HS143 using natural transformation via the uptake signal sequences, as previously described [13].
Polymerase chain reaction (PCR) was used to amplify all twelve ACADM exons, including part of the flanking intron sequences as previously described [11].
Similar(11)
Soil samples were processed for shotgun metagenome sequencing as previously reported12.
Amplicon libraries were prepared and sequenced as previously described17, with primers listed in the Supplementary Table 5.
Other libraries were cloned into bacterial vectors, arrayed individually into the wells of growth trays and sequenced as previously described11,29,30,31. Several analyses were performed before initiating the assembly.
The dendrimer units were built up in a condensation/activation sequence as previously described using a phenol containing two 4-fluorophenylthio groups.
Moreover, we confirmed that transcriptional activation of Runx1 does not affect the integrity of the coding sequence, as previously observed for Runx2.
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