A-G repeat sequences are reported in Additional file 2. In BAP proteins found in wholly-sequenced A. baumannii genomes (Fig. 1), the NH2 region is conserved, the COOH region varies in length because either 2 or 4 copies of the EFG module are present (Additional file 2).
Their sequences are reported in Table 1.
Gene function and primer sequences are reported in Table 1.
In addition to the ubiquitous Radix rubiginosa, we have confirmed the presence of Austropeplea viridis and Radix swinhoei, for the latter of which the ribosomal rDNA sequences are reported for the first time, in North-Thailand.
Primer sequences are reported in Figure S4.
Probe sequences are reported in Table S1.
The widespread occurrence of NISE is becoming increasingly apparent as more genome sequences are reported [1], [3], [62], [63], [64].
Primers and probe sequences are reported in Table 3. Quantitative analysis of gene expression was performed using the Applied Biosystems 7300 Real Time PCR System (Applied Biosystems).
Primers of VE-cad, VEGF165, KDR, uPA and uPAR (all from Eurogentec) were used as the final concentration of 250 nM; their sequences are reported in Table 1.
The total number of misincorporations distributed among the six complementary groups, for ancient and modern sequences are reported in Table 1.
To this aim, a specific duplex Taqman assay was developed amplifying 0.25 ng RNA reverse-transcribed to cDNA in the presence of 0.1 µM each dual labelled probe (HEX/BH1 for actin; and Texas Red/BH2 for 18S rRNA), 0.1 µM and 0.4 µM each forward and reverse primer, respectively for 18S rRNA and actin (sequences are reported in Table S1).
