Exact(14)
Additional loxPsym sequences are inserted in place of deleted non-intronic features and a thinning algorithm ensures that minimum inter-loxPsym site distance is greater than 300 bp.
Ctnnb1fl.(ex3) mice contain a genetically engineered β-catenin gene that loxP sequences are inserted in either side of the exon 3. The peptide encoded by the exon 3 is responsible for degradation of β-catenin.
The advantage of recombineering is that the GFP encoded sequences are inserted into large (30 to 40 kb) genomic DNA fragments cloned into fosmid vectors (data available at http://elegans.bcgsc.bc.ca/perl/fosmid/CloneSearch).bcgsc.bc.ca/perl/fosmid/CloneSearch
Cells (3×105) were plated into 35 mm dishes containing pre-sterilized coverslips for 24 hours, and transfected with 2 µg of plasmids (pEGFP-CAG0/58/200 and pEGFP-CTG200, where repeat sequences are inserted in the 3'-UTR of EGFP) using Lipofectamine™2000 (Invitrogen) according to the manufacturer's instruction.
Exogenous sequences are inserted into specific locations in the virus genome without destroying its infectivity [ 16].
For each of these species, UniProtKB/SwissProt - the manually curated part of UniProtKB - sequences are inserted in the database.
Similar(45)
Aligned sequences were inserted into the SSURef SILVA guide tree using the parsimony insertion function of ARB [59].
Briefly, the codon optimized sequences were inserted into the subgenomic reading frame of a modified DNA plasmid encoding the self-amplifying RNA41.
These sequences were inserted into a movement-deficient version of CPMV RNA-2 which is rescued from cowpea plants (Vigna unguiculota) by inoculation with RNA-1.
Confirmed AtPIP1 4 promoter and cDNA sequences were inserted into pCAMBIA1301 at the front of P35S and the right border to form the genetic complementation union and exclude P35S in the recombinant vector.
The human proinsulin gene cDNA engineered with concensus furin cleavage sequences was inserted into an advanced lentiviral vector that contained CMV early promoter.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com