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As shown in Supplementary Table S4, 4060 (81%) cDNA sequences are formatted on Barley 1 GeneChip and 1300 of them are assigned genetic map positions.
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All genome and protein sequences were formatted using FormatDB.
The resulting sequences were formatted for alignment in the UCSC genome browser.
All D1 6 animal sequences (table S1), except the anthozoan sequences, were formatted as database using the formatdb BLAST utility with default parameters.
The non-overlapping sequences were formatted and treated as two separated sequence contigs.
The first hit was retained and sequences were formatted as per the database.
Gene models, transcript sequences, microarray expression data and gene network data were stored in the MySQL database, and transcript sequences were formatted as a Basic Local Alignment Search Tool BLASTT) database.
These sequences were formatted as databases in a local computer, and the DNA sequences of the mapped markers were used to do similarity searches against the databases by using BLAST software.
The putative conopeptides identified by Barghi et al. (2015) were also added, the redundant sequences of the data set were removed, and the remaining sequences were formatted into the "reference conopeptide database" using formatdb software of BLAST Basic Local Alignment Search Tooll) 2.2.29+ (Altschul et al. 1990).
Accession numbers can be found in Additional File 9. Sequences were formatted in BBEdit Lite 6.1 [ 85] before being aligned using clustalx [ 86] with reduced gap penalties (for pairwise alignments the gap opening penalty was set to 5 and the gap extension penalty to 0.05; for the multiple alignment the gap opening penalty was set to 5 and the gap extension penalty to 0.1).
All ASGARD sequences have been formatted as nucleotide BLAST databases.
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