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General aspects of the engineering of 2Q-recoupling pulse sequences applicable to half-integer spins are discussed, emphasizing the improvements offered from a diversity of supercycles providing enhanced suppression of undesirable AH cross-terms between resonance offsets and rf amplitude errors.
For instance, as often used in species for which no direct species-specific sequence information is available, conserved sequences in specific genes and genomic regions of interest can be used to design primer sequences applicable to a wide variety of organisms [e.g. [ 38]].
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Since our algorithm was originally developed based on discoveries and studies concerning siRNA functionality, its high success rate in shRNA design suggests that most design concerns for siRNA sequences are applicable to shRNA design.
They were also able to recover many motifs from three mixed strains (E. coli, G. metallireducens, and C. salexigens), even when the motif sequences of the genomes of interest overlap substantially, suggesting that PacBio sequencing is applicable to metagenomics.
Consequently, there is a need for robust and low cost amplicon sequencing methods applicable to large populations.
For that reason, the search for specific sequence-enrichment protocols applicable to massive parallel sequencing has been an important goal during these years.
The result is a compact, portable and extensively tested C++11 software framework and set of applications tailored to the demands of next-generation sequencing data and applicable to many other applications.
This strategy is broadly applicable to sequencing applications that benefit from low-cost high-throughput sequencing, but require longer read lengths.
These candidate vaccine antigen sequences may be applicable to any avian or human influenza A virus.
For example, many tools are limited in scope: e.g., OREST [ 38] is only designed for processing mammalian and fungal sequences, not necessarily applicable to other research communities.
It should be noted that the estimated false positive rate from coding sequences would not be applicable to non-coding sequences if we had included scores other than the RNA score from QRNA, such as the COD and OTH scores (COD and OTH scores express the likelihood of being a coding sequence and a non-RNA, non-coding sequence, respectively).
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