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A master schedule is compiled, specifying the sequences and target dates for completion and testing of each component system.
This technique utilizes reverse transcriptase (RT) to convert RNA into complementary DNA (cDNA), a thermostable DNA-dependent DNA polymerase for the amplification of specific target sequences and target specific probes (oligonucleotides) labelled with fluorophores for the detection of amplified DNA (Gibson et al. 1996).
Sequences and target loci of all human and mouse siRNA are detailed in Figure S13.
The ZFR300-coding sequence was modified so as to replace the Zif268 domain with ZifA or ZifB, synthetic variants with published sequences and target sites (Figure 2B) [2B].
We compared the H7-RT-LAMP primer sequences and target sequences of the H7N9 virus.
The green/black triangles indicate the transposon target site sequences and target site duplications.
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Primer sequences and target-specific fluorescence-labelled TaqMan probes were purchased from Applied Biosystems (TaqMan Gene Expression Assays, Supplementary Table S2).
Primer sequences and target-specific fluorescent labeled TaqMan probes used for both genotyping and allele-specific gene expression were purchased from Applied Biosystems (TaqMan SNP Genotyping Assays).
The sequences and targets of ta-siRNAs also tend to be conserved across species.
An improved mcrA probe set included all known mcrA sequences and targeted new variants with explorative probes (Supplementary Table S2).
GATA2 knockout TALENs were designed as described [ 25, 26], and their sequences and targeting site were illustrated in Additional file 1: Figure S1.
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