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Here we demonstrate "Low-Irradiation Generation of High Tesla-SABRE" (LIGHT-SABRE) which works with simple pulse sequences and low power deposition; it should be usable at any magnetic field and for hyperpolarization of many different nuclei.
Raw sequencing reads were trimmed by removing Illumina adapter sequences and low quality bases.
The raw data were trimmed by removing adapter sequences and low quality sequences using CutAdapt (Martin 2011).
Prior to the search, repetitive sequences and low quality regions were masked out of the database at Baylor.
Adaptor sequences and low quality sequence reads were removed prior to assembly.
Small RNA reads were trimmed to remove adapter sequences and low quality reads.
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After removal of terminal adaptor sequences and low-quality data, reads were mapped to the reference human genome (hg19) and aligned using BWA (0.7.12-r1039 46.
Adaptor sequences and low-quality regions in paired-end and mate-pair reads were trimmed using Platanus_trim56 (version 1.0.7) with default parameters.
Trimming of Illumina adaptor sequences and low-quality bases (Q < 20) at the 5′ and 3′ ends of each read was performed by the fastx_clipper program in the FASTX-Toolkit and an in-house C language program.
All reads were trimmed to remove primer sequences and low-quality sequences.
Trimming of vector sequences and low-quality regions from source sequences was performed using commercial software Sequencher 4.9 (Gene Codes, MI, USA).
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