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This novel approach aims to minimize alterations to native BKV polypeptide sequences and expression, potentially allowing maintenance of viral viability.
These include the whole-genome reference assembly for isolate IPO323, genome resequencing of alternate isolates, mitochondrial genome sequences, transcriptome sequences and expression data, and annotations of gene structure and function.
Since genomic integrity is the key to prolonged stability of recombinant CHO cell lines, we characterized piRNA sequences and expression in six CHO cell lines by computational analysis of an existing small RNA sequencing dataset using proTRAC and the published CHO genome as reference.
The sequences and expression patterns of these four genes were investigated to identify the NUDA/GL-1 candidate gene.
By comparing the sequences and expression patterns of different pubescent and glabrous varieties, LOC_Os05g02730, a WUSCHEL-like homeobox gene (OsWOX3B) was identified as the candidate gene.
The tb1 QTL has variable promoter sequences and expression levels in maize and teosinte, while a single amino acid difference in tga1 explains the phenotypic variations in kernel traits.
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C1Chrimson was fused with the C-terminal FLAG-tag sequence, and expression and purification were performed as described above.
Greene GL, Gilna P, Waterfield M, Baker A, Hort Y, Shine J. Sequence and expression of human estrogen receptor complementary DNA.
At the molecular level, adaptive changes in protein sequence and expression impact organismal fitness and, consequently, dictate population dynamics.
Cloning, sequencing and expression study of this gene establishes the functioning of this gene in T. clypeatus.
Here we show that sequence and expression domains of opg, cbfal and a member of the dlx gene family are highly conserved between medaka and higher vertebrates.
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