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The use of Pfam motifs to define gene families in this study reduces the likelihood of such sequences affecting the data, since Pfam motifs are based on multiple sequence alignments of well-studied proteins.
Eleven recombinant Cre proteins were prepared in order to evaluate sequences affecting the uptake and/or activity of cell-permeant enzymes.
In the present study, eleven recombinant Cre proteins were prepared in order to evaluate sequences affecting the uptake and/or activity of the enzyme in cultured cells and if possible to develop more active recombinases.
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Hence, the presence of unidentified repeats in genome sequences affects the quality of gene annotations and annotation-dependent analyses (e.g. microarray analyses).
The mechanism by which the upstream sequences affect the specificity of interaction remains unclear.
It is found above that different peptide sequences affect the peptide assemblies and their properties.
We thus checked whether the presence of these sequences affects the expression level of the recombinant proteins.
Matched 3'-end bases and unique 3' sequences are prerequisites for primer design, because unmatched end bases and 3' sequences affect the progression of Taq polymerase and primer binding, respectively, and can lead to amplification failure.
The modification of gaps in assembled sequences affects the positions in the reads the assemblies are composed of.
Nevertheless, we cannot exclude that the limited number of fossil sequences affected the interpretation of the aDNA data.
The methylation status of CpG moieties within regulatory DNA sequences affects the transcription of the related gene [ 2, 10].
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