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To estimate somatic copy number alteration, we used ReCapSeg (http://gatkforums.broadinstitute.org/categories/recapseg-documentation), which calculated proportional coverage for each target region and then normalized each segment using the median proportional coverage in a panel of normal (PON) samples sequenced with the same capture technology.
The amplified DNA fragments were purified with a GeneElute PCR Clean-Up Kit (Sigma-Aldrich, St Louis, MO) and sequenced with the same primers.
The 16S RNA genes were PCR amplified (with the primers 27F and 1492R [23] ) and sequenced with the same primers and two others (946F and 518R [23]).
Exon fragments of VILIP-1 containing exon and exon-intron junctions were amplified with the primers listed in supplemental Table S1 from cellular genomic DNA and sequenced with the same sets of primers by the Automated DNA Sequencing Facility at Fox Chase Cancer Center.
The multiple anchor primers allowed multiple positions to be sequenced with the same set of query.
PCR products were sequenced with the same primers used in the RT-PCRs.
Similar(32)
To determine the taxonomic affinities of these strains, primers ITS5 and ITS4 [ 21] were used to amplify the approximately 540bp ITS, followed by sequencing with the same primers.
After purification with the Exostar kit (GE Healthcare, Little Chalfont, UK), PCR products were sequenced bidirectionally with the same amplification primers using Big Dye Terminator Kit v3.1 (Life Technologies).
Furst can -and does -- write action sequences with the same vividness as he describes a city like Istanbul.
Several PCR reactions were set-up with MangoTaq and the products were gel-extracted, pooled and sent for sequencing with the same primers used for the amplification.
A translator is used to allow the user to design pulse sequences with the same programming language used in the actual experiment on a real spectrometer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com