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A short stretch of COI has enough information and can be straightforwardly sequenced with a sequencer.
Some PCR products were sequenced with 3730 sequencer (applied biosystem).
DNA was sequenced with Applied Biosystems ABI 3700 sequencer.
The cDNA clones were sequenced with ABI 3730 automated sequencers.
Amplicons were directly sequenced with dye terminators and automated sequencers.
and sequenced with an ABI 377 DNA sequencer.
Positive clones were sequenced with primers T7/SP6 by a DNA sequencer (ABI 3730).
Clones were sequenced with Sequenase (Amersham) and ABI 377 sequencers.
The resulting PCR products were sequenced with the above primers using ABI3700 capillary sequencers.
The libraries were sequenced with 100 bp paired-end reads on an Illumina HiSeq 2500 sequencer.
They were sequenced with adjacent stones for color and graining continuity.
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