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We sequenced up to 24 rRNA clones per positive specimen and assigned taxonomic designations to approximately the species level.
Using non-paired end sequencing chemistry, strains were sequenced up to a depth of 15x and assembled de novo by the 454 Newbler assembler.
Since none of the mollusk ERs sequenced up to now binds E2 either, our data suggest that E2-binding by ER is restricted to vertebrates, implying that vertebrates specifically gained the ability to be regulated by E2 (see Discussion).
We have successfully cloned and sequenced up to 16 tandem barcodes per plasmid, and could routinely clone an average of seven barcodes per plasmid in pilot experiments (data not shown).
RNA-Seq and MethylC-Seq libraries were prepared from two biological replicates (two different individuals of Caucasian female) for each experimental condition, and sequenced up to 75 cycles using the Illumina Genome Analyzer IIx or HiSeq2000.
The publication of the grapevine genome sequence by Jaillon et al. in 2007 [ 1] included the discovery of an ancient hexaploidization event, which also showed clear traces in all the other eudicot genomes sequenced up to that time, but which was absent from the monocot genome of rice.
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It supports the production engineer throughout the entire planning process, departing from part analysis via task sequencing up to the generation of detailed work instructions.
Microbial diversity was assessed by sequencing up to 24 clones per subject.
We analyzed the methylation levels by sequencing up to 20 individual bacterial clones containing bisulfite converted DNA.
The insert was then sequenced with UP and RP primers.
By lifting the gallery level into the air, the architects lead us to anticipate an intuitive sequence up toward the sunlight.
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