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A sample of 114 allelesleles from the Gambian population (one allele from each of 114 independent clinical isolates) was sequenced to enable estimation of smaller sample sizes that would be efficient for detecting positive Tajima's D values (see below).
Where available, DNAs from additional family members were sequenced to enable segregation analysis.
Other protocols offer highly multiplexed single-cell sequencing, nevertheless only a limited number of bases either from the 5'-end [ 30] or the 3'-end [ 34] are sequenced to enable mainly gene expression studies.
Two samples, 577_1_Emelia and 577_2_Raman, were originally sequenced to enable the characterisation of elephant endotheliotropic herpesvirus (EEHV) [ 12], which is associated with a life-threatening haemorrhagic disease in Asian elephants [ 6].
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The CHESS sequence was combined with MR imaging sequences to enable visualisation of the water distribution in real time in one-dimension, or as a time-averaged measurement in two dimensions.
Enrichment was followed by de novo sequencing to enable an unbiased realization of all possible sequences in the sample.
However, shunting requires shunt donor and acceptor sequences to enable scanning ribosomes to bypass mRNA sequences in the 5' UTR, none of which were found in the Ring1B 5' UTR.
Restriction sites for BamHI and HindIII were included at respectively the 5'and 3'end of the coding sequences to enable cloning in the corresponding restriction sites of expression vector pET15new.
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