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He had his tumor's genome sequenced, to check for special vulnerabilities.
The PCR product was cloned into pTA2 (Toyobo, Osaka, Japan) and sequenced to check for PCR errors.
PCR products were sequenced to check their correspondence with the gene under study.
Clones were sequenced to check for any mutation that might have been misincorporated during the amplification.
The PCR product was again sequenced to check the integrity of the construct.
PCR amplifications were performed with the high fidelity Pfu polymerase (Promega) and constructs were sequenced to check for the absence of mutations.
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Prior to large-scale Solexa sequencing, we performed clone sequencing to check the outcome of MMSDK.
Entry clones for ChR2 and yfp were examined by sequencing to check that no errors were incorporated during the PCR amplifications.
After the transfection, the RNA extracts were submitted for RT-PCR, cloning and sequencing to check whether the expressed RNA encompasses both the vector part and the inserted sequence, an evidence that the transfected sequence has successfully expressed in our system.
Therefore, as pointed out in [26], empirical methods are almost always applied, relying on the outputs of MCMC samplers and diagnostics computed from the produced sequence to check convergence.
We randomly picked some scats (that showed new haplotypes after sequencing) and repeated the entire process from extraction to sequencing, to check the validity of new haplotypes.
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