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As the DNA is being sequenced, modification to bases result in a delay in the called bases, called the interpulse duration ratio (IPD) [ 39].
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This sequence modification had no effect on the silencing efficiency of the siRNA.
How and why the sequence modification of miRNA precursors affect their silencing property are yet to be fully understood.
Here we report a novel system to select sgRNA with high efficacy for DNA sequence modification by a luciferase assay.
In the current study, we attempted to optimize peptide bioactivity by sequence modification and assess the antimicrobial activities.
Nucleotide sequence modification through single base editing in animals is emerging as an important player in tumorigenesis.
Rational approaches are the molecular modification (site direct mutation) of the ligninolytic enzyme sequence using prior structural information as foundation for the specific sequence modification.
Our data suggest that this sequence modification coupled with structural modification differentially recruits human TLR8 over TLR7, and could have potential application in antiviral therapies.
The G-DOPA-G N and C terminal sequence modification peptide can be used as immobilization strategies of antimicrobial peptides on titanium alloy surfaces.
Circular dichroism and nuclear magnetic resonance spectra of all engineered variants demonstrate that they maintain their native conformations despite substantial sequence modification.
Unfortunately, the conventional methods used to create random circular permutation libraries cause significant sequence modification at new termini of circular permutants.
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