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As genome and EST coverages increase and additional diatom genomes are sequenced, evidence for the GPCR signaling pathway in these organisms will likely become more complete.
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Sequencing evidence from gene boundaries was consistent with integration.
Phylogenetic and sequencing evidence from gene boundaries was consistent with integration of filoviruses in mammalian genomes.
In addition, we further investigated the novel 57 SVs without either sequencing evidence or database supporting evidence.
For each gene's deep sequencing evidence of the 30 samples, each sample was normalized using the RPKM value [ 61].
For example, the ECO:0000224 term 'SOLiD sequencing evidence' can be created as an output of the OBI 'planned process' subclass 'SOLiD sequencing'.
Where possible, ECO makes use of the OBI ID as definition provenance (for example, in the definition of 'ECO:0000224' SOLiD sequencing evidence).
RT-PCR on mRNA extracts from strain IPAP/MA/86/LEM898 followed by sequencing evidenced that the CL A2 gene is functional.
In contrast, next-generation sequencing-based transcriptome determination defines genes directly from experimentally derived mRNA sequence evidence.
There are many excavated sequences but the numbers of points from those sequences are rather modest (perhaps unlike invertebrate fossils), nor is their co-occurrence in stratified sequences evidence of historical continuity in a set of points.
In the best-studied cases, the bacterial Lyme disease spirochaete Borrelia, and the sleeping sickness protist Trypanosoma brucei, the genome sequence evidence for cassette recombination is strong, but the process is not as well understood as the yeast mating-type switches.
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