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However, as more samples are sequenced, errors from individual assemblies are likely to accumulate, which could seriously affect the isoform abundance estimation and result in unreliable (or even misleading) differential analysis results.
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Kelley, D.R., Schatz, M.C. & Salzberg, S.L. Quake: quality-aware detection and correction of sequencing errors.
Singletons are sometimes removed due to possible problems with sequencing errors associated with Illumina and other next-generation sequencing platforms19.
To further reduce sequencing errors, sequences that were within 2 bp of a more abundant sequence were merged using the pre.cluster command.
Smith, T., Heger, A. & Sudbery, I. UMI-tools: modeling sequencing errors in Unique Molecular Identifiers to improve quantification accuracy.
We identify sites that are prone to sequencing errors, including thousands present in publicly available variant databases.
For many alignment and assembly applications, sequencing errors can confound downstream analyses.
Presumed sequencing errors in the published cDNA sequence of EPHA1 were identified in exons 10 and 11.
When items appeared in nonconsecutive locations in Experiment 2, the advantage of the spatial presentation on sequencing errors disappeared.
Sequencing errors are not evenly distributed along sequence reads [14].
Conversely, they might be very often results of sequencing errors.
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