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The sequenced ends of the BAC clones from the CHORI105 library [ 11] were mapped to the contigs based upon BLAST [ 12] analysis.
An important step forward in dissecting the Musa genome was made by Cheung and Town (2007), who sequenced ends of more than 6,000 BAC (Bacterial Artificial Chromosome) clones [ 12].
Paired-end mapping (also called read-pair) approaches inherently have uncertainty in breakpoint resolution, due to uncertainty in the distance between sequenced ends and the possibility of read mismapping (Korbel et al., 2009; Medvedev et al., 2009).
We sequenced ends of cores using an Illumina HiSeq2500 sequencing platform to obtain 339 587 million 25-nt single-end reads for each tissue type, 66%% of which mapped to unique genomic positions, yielding 46-72-fold 46-72-fold 46-72-folde genome for eacoveragee type (Table 2), which was sufficient tofestheatentireeosome positioningenome
Additionally, 39,605 sequenced ends of the CHORI-105 BAC library (produced by Pieter de Jong at the Children's Hospital Oakland Research Institute and sequenced by TIGR), consisting of the TARBAC (avg. length 75 kb) and EPIFOS (avg. length 35 kb) libraries, were obtained from the GSS database at NCBI.
Second, the complete sequence of the plasmid insert was acquired by alignment of the sequenced ends against the genome sequence of the strain ISA1307 available in the PEDANT database (http://pedant.helmholtz-muenchen.de/genomes.jsp Category=fungal) [ 37].
Similar(46)
Consequently we set out to have a glimpse of the genomic structure by sequencing ends of fosmid clones.
CT sequenced BAC ends.
Additionally, 1577 BAC clones were end sequenced; 2505 BAC end sequences (80% of BACs) were obtained.
The sequence ends with a violently plunging octave figure.
The sequence ends with the player turning the gun on his captors.
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