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The sequences in each library were divided into separate groups on the basis of their sequencing direction (3′ or 5′ ESTs) where sequencing direction data were available.
Tissues, dbEST library identifier, sequencing direction, and library descriptions are provided.
Stacked reads i.e. redundant reads sharing the same start and end coordinate, sequencing direction, and sequence were merged into one.
To generate features that are discriminative of the sequence direction, we model the peptide as a directed graph.
In the same way, utilizing the zero-sequence direction element, the limited wide area backup protection can be established based on the pilot channel.
Skewed sequencing direction was highly associated with errors.
For unigenes with sequence direction, sequences from the 5' end to the 3' end were provided.
For those without sequence direction, we obtained sequences from assembly software.
The best-aligning results were used to determine the sequence direction of the unigenes.
The best alignment results were used to determine the sequence direction of unigenes.
The best hits were used to decide on sequence direction of transcripts.
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