A total of 17,657 genes and 75,392 transcripts were obtained at sequencing depth of 200M.
With sufficient sequencing depth, tumor-specific mutations, which may contribute to pathogenesis, can be detected.
TG performed sequencing depth simulations.
Another potential contributing factor is sequencing depth.
Coverage equals sequencing depth per individual.
However, the sufficient sequencing depth remained obscure.
Twenty of them are of sequencing depth 1×; 20 of them are of sequencing depth 2×; 20 of them are of sequencing depth 3×; 20 of them are of sequencing depth 4×; 5 of them are of sequencing depth 5×; the remaining 15 are of sequencing depth 11×, 12×, …, 25×.
One-hundred species are randomly picked from 18 genera; 20 of them are of sequencing depth 1×; 20 of them are of sequencing depth 2×; 20 of them are of sequencing depth 3×; 20 of them are of sequencing depth 4×; 20 of them are of sequencing depth 6×, 7×,…, 25×, respectively.
Figure 7 shows a plot of sequencing depth of individual sequences for these three datasets.
Each sample should meet the following criteria: i. average sequencing depth of WGS ≥ 30×, average sequencing depth of WES ≥ 100×; ii.
