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SymBio Corporation (Menlo Park, CA) amplified the cDNA clones using rolling circle amplification, and then sequenced about 4,000 cDNA clones per library using a MegaBASE 4000 sequencer (GE Healthcare, Little Chalfont, UK).
Before the project, scientists had only sequenced about 20 species in the microbiome.
Although the rice genome was completely sequenced about 15 years ago, the function of most rice genes is still unknown.
His lab isolated and sequenced about 500 bases from a gene often used to tell one type of bacterium from another.
Thus far, she has sequenced about 1000 DNA bases, which she has compared with the previously reported sequence of HIV-1 extracted from a frozen blood sample from 1959.
They sequenced about 500 bases from each animal, studied the differences, and firmly established that the donkey family tree has two branches; one in Asia and the other in Africa.
Similar(32)
We re-sequenced about 15 30 kilobases (kb) from each of the four genes using long-read Sanger sequencing, focusing on segments containing mutations that have been documented as medically important, or containing novel amino acid changes on the human lineage (Table 1).
Most of contemporary dose-seeking phase I trials remain guided by the "modified Fibonacci sequence" (about 50%, Table 1).
Individual mutant chromosomes were sequenced at about 20-fold coverage.
Dicer generates short RNA sequences of about 22-nucleotides.
The full activation sequence took about 220 250 ms.
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