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Additional analysis provided evidence for sequence-targeted cleavage, including putative targeting sequences, as one mechanism of degradation for several hundred transcripts in the maturing oocyte.
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Alternatively, NGS sequencers can be used to sequence targeted genomic regions from a very large number of samples arranged in multidimensional pools.
All HA-RanBPM constructs contain a point mutation in the sequence targeted by the RanBPM siRNA (except for ΔC4 which lacks the targeted sequence), as previously described (Atabakhsh et al., 2009).
Understanding how to sequence targeted therapies for metastatic renal cell carcinoma (mRCC) is important for maximisation of clinical benefit.
Another hurdle for the use of CRISPR Cas9 technology is the possibility of off-target edits that might arise if editing components bind to genomic regions that have high similarity to the sequence targeted by the RNA guide.
An antisense (AS) oligonucleotide (ODN) sequence targeted at monoamine oxidase-B (MAO-B) was utilized to attenuate MAO-B activity prior to MDMA administration.
The sequence targeted for capture was derived from the West Nile Virus (WNV) RNA genome and selected on the basis of exhibiting minimal secondary structure formation.
Even if the sequence targeted for removal by CRISPR is present in multiple places in the genome, Greene says, researchers need to consider only those lying next to the PAM sequences.
Even in the initial discovery of the anti-phage role of CRISPR-Cas systems in bacteria, it was evident that phages had means of bypassing that immunity a phage with a single point mutation in the 30-nt sequence targeted by the crRNA2, or in the flanking proto-spacer adjacent motif (PAM 6 could freely infect the CRISPR-immunized bacterial host.
Lead Accelerator is the big product launch, allowing businesses to divide their audiences into different segments, and then to deliver ads and content in the right sequence, targeted to each group.
The sequence targeted by each TaqMan probe is given in Table S3.
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