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The total sequence yield was 39.1 million reads.
Recently, Illumina has released a newer version of their pipeline, SCS v2.8/OLB v1.8, which promised an increase in cluster detection efficiency, and thus sequence yield.
Second, the duplicate removal process also reduced the usable sequence yield from each experimental run by nearly half, although this is an overestimation due to the naïve nature of the method.
In essence, the proportion of sequences with intact barcode stayed roughly the same in all samples analysed (Fig. 4a and Table 2), indicating that a net increase in sequence yield translates directly into an increased yield of barcoded, and therefore usable sequences.
Overall sequence yield was expected to be directly proportional to the number of FOV.
Also, the sequence yield for amplicon 18 was relatively low in comparison to that of the other amplicons.
Similar(31)
By the calculations above, taking (srightarrow 1) along this sequence yields the desired strict inequality.
The DFT of this sequence yields l complex coefficients in the frequency domain ( X orig [ j ], j = 1, …, l ).
Protein expressed from the engineered sequence yielded crystals that belong to space group P21.
This sequence yielded image volumes of 50 slices, 3 mm thick, oriented to exactly match the PD/T2-W sequence.
The sequence yields shown in Fig. 1a were analysed by ANOVA followed by Bonferroni post-hoc tests.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com