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Since the sequence was conducted on multiple occasions, the stuntmen were coated with gel containing cationic polymers.
The comparison of overall plasmid sequence was conducted by WebACT and Geneious Pro (Version 5.0.1, Biomatters Limited, Auckland, New Zealand).
The sequence was conducted according to literature [ 10].
Trimming of the assembled C-mos sequence was conducted using Cross_Match [ 23].
The first sequence was conducted without pH control using glucose as a carbon source.
Gene prediction of the finished DSW-6 genome sequence was conducted using Glimmer 3.0 (Delcher et al. 2007).
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Transcriptome sequencing was conducted using Illumina Hiseq2000 sequencer.
The sequencing was conducted on a MiSeq sequencer with 50 cycles of library sequencing.
Sequencing was conducted on an ABI 3730 automated sequencer (Invitrogen, Shanghai, China).
Sequencing was conducted on an ABI 3130XL automated sequencer (Applied Biosystems).
The sequencing was conducted at Penn State University using an FLX model 454 DNA sequencer (Roche Diagnostics).
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