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Each reaction can cleave the sample sequence to completion at all loci wherever a specific base is found.
In our hands, MboII was an inefficient cutter that never digested wild-type ER sequence to completion.
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was the only plant species that had been sequenced to completion, although rice chromosome 1 had been completed and the rest of the rice sequence was soon published [ 2, 3].
All sequencing was performed using terminator-based cycle sequencing with BigDye chemistry (Applied Biosystems, Foster City, CA, USA) on an ABI 3730 or 373 DNA sequencer (Applied Biosystems) at Shanghai Sangon or Takara Biotechnology Co. Amplicons were sequenced to completion by primer walking.
About the half of OTUs (165 out of 346) were sequenced to completion.
Genomes of 50 different species of the subclass Actinobacteridae including multiple strains of the same species have been sequenced to completion (Table S1) [61].
Seven Y. pestis genomes have been sequenced to completion, along with five other Yersinia sequences.
The genome of E. sibiricum strain 255-15 has been sequenced to completion by DOE's Joint Genome Institute.
Among the genomes that had earlier been sequenced to completion, the three ssp.
The dataset included 58 genomes in total, 39 of which were sequenced to completion.
Both strands of the ITS1 were sequenced to completion using primers 1645F and RotIIR.
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