Sentence examples for sequence terminator from inspiring English sources
Plasmid pXen-13 containing the luxCDABE operon was modified to pLKMp by insertion of a Neisseria specific promoter sequence, terminator sequence, kanamycin resistance gene, DNA uptake sequence and two DNA fragments homologous to a non-coding region of the FAM20 genome (Figure 1A and Table 1).
The pPIC-PST vector consisted of T. reesei cellobiohydrolase I (cbh1) promoter, signal sequence, terminator, and a zeocin-resistance gene.
The transcriptional terminators were characterised by comparison between transcripts level using primers upstream of the putative terminator sequence (Terminator) and downstream of the terminator sequence (External).
The DNA sequencing was done using BigDye 3.1 sequencing terminator kit and ABI 3730xl DNA analyzer (Applied Biosystems Inc. USA).
The PCR products were sequenced using the ABI BigDye cycle sequencing terminator kit (Applied Biosystems) according to the manufacturer's protocol.
Mutation analysis was carried out using dHPLC (Transgenomic WAVE Nucleic Acid Fragment Analyzer System Model 3500HT) and samples with aberrant patterns were further analyzed by direct sequencing (Terminator Cycle Sequencing Reaction Kits version 3.1, ABI Prism 3100 Genetic Analyzer; Applied Biosystems).
The inserts were subjected to cycle sequencing (BigDye terminator v3.1 Cycle Sequencing kit, Applied Biosystems, USA).
The three purified promoter, coding sequence and terminator fragments were assembled by overlap extension PCR (OE-PCR).
The U6 promoter, Fto hairpin sequence and terminator sequences were subcloned into the multicloning site of the pAAV-MCS backbone (Stratagene) to generate the corresponding AAV-shRNA vectors.
The MSTN promoter, EGFP coding sequence, MSTN terminator were assembled using ABI-REC.
PCR products were gel-purified (Qiagen kit), cloned into pGEM-T Easy vector (Promega, USA) and sequenced using BigDye Terminator sequencing method (ABI, USA).
