Exact(1)
To assign gene ontology terms to each annotated sequence, successful blast hits were mapped and annotated using Blast2GO for the entire assembled transcriptome with the annotation cut-off threshold set to 55 and the GO level weighting set to 5. The single lane of Illumina HiSeq2000 produced close to 128 million paired-end reads (2 × 101 bp).
Similar(59)
Only those sequences successful for both RBH searches were assigned orthologs (figure 1, route 2).
Because the repetitive nature of centromeric α-satellite DNA has thus far disallowed complete sequencing, successful annotation of transcriptional motifs that may exist in human centromeres remains to be accomplished.
Percentage of high quality sequences from successful sequences; percentage of unique sequences of the high quality sequences; percentage of unique sequences The processed sequences were subjected to cluster analysis using Vector NTI to evaluate sequence redundancies.
Radiation hybrid (RH) mapping is an important tool in the construction of high-resolution physical maps, which are key to efficient sequencing and successful genome sequence assembly [ 1- 5] and the construction of comparative maps between species [ 6- 8].
Now the probability of a sequence of successive successful updates of length is positive.
The resulting 1,110 BAC clones were end-sequenced, with successful sequence being acquired from 851 clones.
Although the high% AT vs.% GC ratio of this genome poses challenges for all high-throughput sequencing platforms, a successful sequencing library was constructed, sequenced and assembled through protocol optimizations.
Plasmids were isolated using the Wizard plasmid purification kit (Promega), and Sanger sequencing confirmed successful insertions of sequences to vectors.
It started a long sequence of successful Soviet Venera missions.
This makes prediction of the full tmRNA sequence less successful.
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