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During one measurement sequence, standards were randomly injected to compensate for possible trends.
Target sequence standards for evaluation are cloned into pCR2.1-TOPO (Invitrogen, Carlsbad, CA, USA) by using PCR amplification of cDNA templates obtained by reverse transcription (RT) of extracts from infected, cultured cells or by assembly of overlapping synthetic polynucleotides.
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A RPK of 60 represents an average sequence depth of 3; the human genome sequence standard for 99% accuracy (http://www.genome.gov/10000923).gov/10000923
Although this was considered a large EST set by pre-next-generation sequencing standards, it constitutes a relatively small collection given today's sequencing technologies.
ESTs shorter than 100 bp were removed from the data set, as were contaminant sequences, such as E. coli, common vectors, and sequencing standards.
The reaction parameters of the multiple reaction sequences, standard potentials, diffusion coefficients, rate constants and saturation coverage, were calculated.
In contrast, almost complete collections of isogenic deletion mutants are available in the BY4743 sequenced standard laboratory genetic background [ 23].
Compared to sequencing standard barcode markers, the designed assay is much faster, less labor-intensive, and hence much cheaper.
To ensure that the new multiband sequence was robust in our older population, we acquired both sequences (standard and multiband) on a subset of participants (N = 76).
The strain FGSC 2489 is the sequenced standard laboratory strain that has been developed through an extensive backcrossing in the laboratory [ 29].
Following sequencing, standard PhiC31-mediated transformation procedures were used to obtain transgenic Drosophila lines carrying UAS-Rack1 on a 65B2 platform chromosome (Bestgene).
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