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The N-terminal 62 residues of NS1 and NS2 are identical in sequence, splicing then generates a shift in the ORF such that the remaining residues are translated from a different reading frame (Alamgir et al., 2000).
This process detects sequence splicing and redundancy to acquire the longest sequences and group them into clusters.
The aim of this study was to conduct an initial analysis of sequence, splicing and expression of the CYYR1 mRNA in human NE tumors.
Finally, unigenes from the two libraries were pooled and processed to remove sequence splicing and redundancy to generate a nonredundant unigene set.
After further processing for sequence splicing and redundancy removal, 110,586 unigenes were generated, with a mean length of 922 bp.
The aim of this study was to analyze the sequence, splicing and expression of the CYYR1 gene in human neuroendocrine tumors.
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This sequence (3' splice site) is recognized by the splicing factors U2AF large and small subunits and plays important roles in regulating splicing efficiency and alternative splicing [11], [12], [13], [14].
In addition, we considered only gene related SNPs, for our purposes defined as either in coding sequence, splice site or mRNA 5' and 3' UTRs, because these directly implicate a specific gene.
Contigs were individually inspected for low-quality sequence, splice variants and chimeras, before ESTs were reassembled.
Thorstenson and colleagues focused their single nucleotide polymorphism (SNP) discovery on the coding sequence, splice sites and 5' upstream sequences.
The IRES sequence was preceded either by the GFP sequence (see Figure 3A2), or by the Munc18-1 splicece (splice variant b), in wild-type or mutated form.
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