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About 200-base genomic regions around the SNPs and indels called by the mapping programmes were amplified by PCR and sequenced on a capillary sequencer with the Sanger method using the commercial sequence service of MACROGEN (Tokyo, Japan).
As part of one such initiative, the members of the Pistoia Alliance [2] have mutually agreed to define and document the standards for a secured sequence service.
The feeding team gained written informed consent and then randomised women immediately after hospital discharge using a website randomisation sequence service set up by an independent statistician.
PCR products were purified using the QIAprep Spin Miniprep Kit Qiagen Ltd , UK) according to the manufacturers instructions and sent to a commercial sequence service provider (MWG Biotech, Germany) for sequencing.
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All sequencing was performed commercially using an Applied Biosystems 3730 DNA sequencer by The Sequencing Service, University of Dundee.
The verified amplicons were purified and sequenced directly by ABI3730XL sequencer in Macrogen sequencing service, Korea [ 5].
Sequencing was performed using ABI Big Dye V3.1 and resolved on an automated sequencer at Macrogene Inc. (Korea) and the University of Canterbury sequencing service (New Zealand).
A new sequencing service could change the face of human genomics.
This past summer Illumina announced its own full-genome sequencing service for $48,000.
The miRNA sequencing service was carried out at the Centre for Genomic Regulation (CRG), Barcelona, Spain.
While most sequencing companies sell machines to labs, which then do their own sequencing, Complete Genomics offers a sequencing service.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com